Analysis of the Genome Sequence and Prediction of B-Cell Epitopes of the Envelope Protein of Middle East Respiratory Syndrome-Coronavirus.
Identifieur interne : 005440 ( Main/Exploration ); précédent : 005439; suivant : 005441Analysis of the Genome Sequence and Prediction of B-Cell Epitopes of the Envelope Protein of Middle East Respiratory Syndrome-Coronavirus.
Auteurs : Qian Xie ; Xiaoyan He ; Fangji Yang ; Xuling Liu ; Ying Li ; Yujing Liu ; Zhengmeng Yang ; Jianhai Yu ; Bao Zhang ; Wei ZhaoSource :
- IEEE/ACM transactions on computational biology and bioinformatics [ 1557-9964 ]
Descripteurs français
- KwdFr :
- Biologie informatique (), Coronavirus du syndrome respiratoire du Moyen-Orient (génétique), Coronavirus du syndrome respiratoire du Moyen-Orient (immunologie), Déterminants antigéniques des lymphocytes B (), Déterminants antigéniques des lymphocytes B (immunologie), Humains, Infections à coronavirus (virologie), Modèles moléculaires, Protéines de l'enveloppe virale (), Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (immunologie).
- MESH :
- génétique : Coronavirus du syndrome respiratoire du Moyen-Orient, Protéines de l'enveloppe virale.
- immunologie : Coronavirus du syndrome respiratoire du Moyen-Orient, Déterminants antigéniques des lymphocytes B, Protéines de l'enveloppe virale.
- virologie : Infections à coronavirus.
- Biologie informatique, Déterminants antigéniques des lymphocytes B, Humains, Modèles moléculaires, Protéines de l'enveloppe virale.
English descriptors
- KwdEn :
- Computational Biology (methods), Coronavirus Infections (virology), Epitopes, B-Lymphocyte (chemistry), Epitopes, B-Lymphocyte (immunology), Humans, Middle East Respiratory Syndrome Coronavirus (genetics), Middle East Respiratory Syndrome Coronavirus (immunology), Models, Molecular, Viral Envelope Proteins (chemistry), Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology).
- MESH :
- chemical , chemistry : Epitopes, B-Lymphocyte, Viral Envelope Proteins.
- chemical , genetics : Viral Envelope Proteins.
- chemical , immunology : Epitopes, B-Lymphocyte, Viral Envelope Proteins.
- genetics : Middle East Respiratory Syndrome Coronavirus.
- immunology : Middle East Respiratory Syndrome Coronavirus.
- methods : Computational Biology.
- virology : Coronavirus Infections.
- Humans, Models, Molecular.
Abstract
The outbreak of Middle East respiratory syndrome-coronavirus (MERS-CoV) in South Korea in April 2015 led to 186 infections and 37 deaths by the end of October 2015. MERS-CoV was isolated from the imported patient in China. The envelope (E) protein, a small structural protein of MERS-CoV, plays an important role in host recognition and infection. To identify the conserved epitopes of the E protein, sequence analysis was performed by comparing the E proteins from 42 MERS-CoV strains that triggered severe pandemics and infected humans in the past. To predict the potential B cell epitopes of E protein, three most effective online epitope prediction programs, the ABCpred, Bepipred, and Protean programs from the LaserGene software were used. All the nucleotides and amino acids sequences were obtained from the NCBI Database. One potential epitope with a suitable length (amino acids 58-82) was confirmed and predicted to be highly antigenic. This epitope had scores of >0.80 in ABCpred and level 0.35 in Bepipred programs. Due to the lack of X-ray crystal structure of the E protein in the PDB database, the simulated 3D structure of the E protein were also predicted using PHYRE 2 and Pymol programs. In conclusion, using bioinformatics methods, we analyzed the genome sequence of MERS-CoV and identified a potential B-cell epitope of the E protein, which might significantly improve our current MERS vaccine development strategies.
DOI: 10.1109/TCBB.2017.2702588
PubMed: 28574363
Affiliations:
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MERS-CoV was isolated from the imported patient in China. The envelope (E) protein, a small structural protein of MERS-CoV, plays an important role in host recognition and infection. To identify the conserved epitopes of the E protein, sequence analysis was performed by comparing the E proteins from 42 MERS-CoV strains that triggered severe pandemics and infected humans in the past. To predict the potential B cell epitopes of E protein, three most effective online epitope prediction programs, the ABCpred, Bepipred, and Protean programs from the LaserGene software were used. All the nucleotides and amino acids sequences were obtained from the NCBI Database. One potential epitope with a suitable length (amino acids 58-82) was confirmed and predicted to be highly antigenic. This epitope had scores of >0.80 in ABCpred and level 0.35 in Bepipred programs. Due to the lack of X-ray crystal structure of the E protein in the PDB database, the simulated 3D structure of the E protein were also predicted using PHYRE 2 and Pymol programs. In conclusion, using bioinformatics methods, we analyzed the genome sequence of MERS-CoV and identified a potential B-cell epitope of the E protein, which might significantly improve our current MERS vaccine development strategies.</div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="He, Xiaoyan" sort="He, Xiaoyan" uniqKey="He X" first="Xiaoyan" last="He">Xiaoyan He</name><name sortKey="Li, Ying" sort="Li, Ying" uniqKey="Li Y" first="Ying" last="Li">Ying Li</name><name sortKey="Liu, Xuling" sort="Liu, Xuling" uniqKey="Liu X" first="Xuling" last="Liu">Xuling Liu</name><name sortKey="Liu, Yujing" sort="Liu, Yujing" uniqKey="Liu Y" first="Yujing" last="Liu">Yujing Liu</name><name sortKey="Xie, Qian" sort="Xie, Qian" uniqKey="Xie Q" first="Qian" last="Xie">Qian Xie</name><name sortKey="Yang, Fangji" sort="Yang, Fangji" uniqKey="Yang F" first="Fangji" last="Yang">Fangji Yang</name><name sortKey="Yang, Zhengmeng" sort="Yang, Zhengmeng" uniqKey="Yang Z" first="Zhengmeng" last="Yang">Zhengmeng Yang</name><name sortKey="Yu, Jianhai" sort="Yu, Jianhai" uniqKey="Yu J" first="Jianhai" last="Yu">Jianhai Yu</name><name sortKey="Zhang, Bao" sort="Zhang, Bao" uniqKey="Zhang B" first="Bao" last="Zhang">Bao Zhang</name><name sortKey="Zhao, Wei" sort="Zhao, Wei" uniqKey="Zhao W" first="Wei" last="Zhao">Wei Zhao</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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